The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
One of virulence factors produced by Staphylococcus aureus is staphylokinase (SAK), which enhances their proteolytic activity leading to tissue damage and improving bacterial invasiveness. In the present study we estimated the ability to produce staphylokinase by 95 S. aureus reference strains and clinical isolates from the airways of cystic fibrosis patients, from skin lesions and from infected bones...
The capsular polysaccharide (CPS) plays important role in Klebsiella spp pathogenesis. Capsular types Kl and K.2 of Klebsiella pneumoniae are considered most virulent for humans. The capsule biosynthesis region flanking genes galF and gnd from clinical isolates and reference strains of K. pneumoniae were screened for polymorphism. Nucleotide sequence analysis of galF and gnd revealed a high heterogeneity...
The mannosylated lipoarabinomannan (ManLAM) from mycobacterial species possesses strong immunomodulatory effects. Here we examined the ability of Mycobacterium tuberculosis ManLAM to interfere with the apoptotic response of mouse monocyte cell line, RAW 264.7 infected with Mycobacterium bovis BCG Danish strain. Incubation of BCG-infected monocytes with ManLAM decreased production of NO and the numbers...
A number of Enterococcus strains with high-level inducible resistance to vancomycin have been identified, and the relative incidence of these strains has increased significantly in the last years. The first outbreak caused by vancomycin-resistant enterococci in Poland was reported in 1999. Vancomycin-resistant Enterococcus faecium is known for its propensity to cause infections which are difficult...
The strain of Escherichia coli K-12 with high aspartase activity was irradiated with UV. After mutagenesis and selection, the mutant B-715 was isolated which was 4-times more active in L-aspartic acid biosynthesis than parental K-12 strain. The highest productivity was achieved while the strain was cultivated in the ammonium fumarate medium in 37°C for 18-30 hours. It was found that better results...
The aspartase overproducing mutant B-715 was used as a donor of the aspartase gene for further construction of the aspartase-hyperproducing strains by molecular cloning. In preliminary experiments activity of transformants and their efficiency in L-aspartic acid biosynthesis were compared. The conditions for recombinant strain multiplication, biomass activation and L-aspartic acid biosynthesis were...
A β-mannosidase was purified from Phlebia radiata grown in a medium containing wheat bran or galactomannan as a carbon source. Maximal activity was observed at pH 5.5 and at 50°C. Highly purified isoforms of β-mannosidase (GM-1, GM-2) isolated from media containing galactomanan and (OT-1) media with wheat bran were obtained by means of column chromatography on Q-Sepharose, SP-Sepharose and chromatofocusing...
Pseudomonas fluorescens BM07 was characterized as a producer of cold-induced biopolymer by decreasing the temperature down to as low as 10°C. It was previously shown that the synthesis of BM07 biopolymer was inhibited at 30°C. The present study was conducted to investigate the biosorption of mercury (Hg²⁺) ions on the BM07 cells grown on Ml minimal medium at two temperatures (10°C and 30°C). The effects...
Wastewater treatment based on activated sludge is known to be one of the most effective and popular wastewater purification methods. An estimation of microbial community variability in activated sludge allows us to observe the correlation between a particular bacterial group's appearance and the effectiveness of the removal of chemical substances. This research is focused on microbial community temporal...
Microbial contamination of a water distribution system was examined. The number and the taxonomy of non-pigmented and pigmented heterotrophic bacteria (HB), number of bacteria (Pseudomonas sp., Enterococcus sp., Campylobacter sp., Yersinia sp., representatives of the Enterobacteriaceae, coagulase-positive staphylococci, and C. perfringens) in the bulk water phase, biomass of zoogloeal aggregates of...
Bacillus subtilis strains (CM1-CM5) isolated from culturable (cowdung microflora were investigated for indole-3-acetic acid (IAA) production in nutrient broth (NB). All the strains tested produced IAA in NB; albeit in very low concentrations (0.09-0.37 mg/l). The addition of L-tryptophan (0.1-1.0 g/l) into NB substantially enhanced IAA production (6.1-31.5 folds) indicating that L-tryp-tophan was...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.